lucigen 40300-4 40 rxns CloneSmart® LCAmp Blunt Cloning Kit 40300-4

产品价格: < 面议
产品型号:40300-4
 牌:其它品牌
公司名称:北京中北林格科技发展有限公司
  地:北京昌平
发布时间:2020-01-14
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产品简介

北京中北林格供应lucigen 40300-4 40 rxns CloneSmart® LCAmp Blunt Cloning Kit,中北林格是lucigen总代理,提供lucigen产品货号报价查询。其他包括epicentre总代理,cellscript总代理,Biosearch technologies总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。lucigen 40300-4 40 rxns CloneSmart® LCAmp Blunt Cloning Kit

产品详细信息

lucigen 40300-4 40 rxns CloneSmart® LCAmp Blunt Cloning Kit
产品简介:北京中北林格供应lucigen 40300-4 40 rxns CloneSmart® LCAmp Blunt Cloning Kit,中北林格是lucigen总代理,提供lucigen产品货号报价查询。其他包括epicentre总代理,cellscript总代理,Biosearch technologies总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。</p>、

产品名称:
CloneSmart® LCAmp Blunt Cloning Kit
货号:40300-4
规格:40 rxns
存储温度:-20º C
品牌:lucigen</p>
供应商:中北林格</p>
产地:us</p>
发货地:北京</p>

lucigen 40300-4 40 rxns CloneSmart® LCAmp Blunt Cloning Kit 产品描述

CloneSmart® Cloning Kits (pSMART® Vectors)

 

产品说明书

 

  • Convenient, pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation.
  • Transcription terminators flanking cloning site stabilize otherwise toxic inserts.
  • Choice of copy number and antibiotic resistance.
  • Optimized for use with E. cloni® 10G electrocompetent and chemically competent cells (sold separately)
  • 方便的预处理载体消除了消化,凝胶纯化和去磷酸化的需要。
  • 位于克隆位点两侧的转录终止子稳定了有毒的插入片段。
  • 选择拷贝数和kang生素抗性。
  • 优化用于  E.cloni®10G  电感受态和化学感受态细胞(单独出售)
 

Clone quickly and facilitate downstream applications

Lucigen’s unique cloning kits with pre-processed vectors and highly competent cells eliminate hours of tedious preparation and enable efficient cloning. Ligation can be completed in as little as 30 minutes with no clean-up needed; only a brief heat denaturation is necessary before transformation. Downstream manipulation (e.g, mutagenesis, subsequent insert addition) is facilitated by the minimal vector size (1.7-2.0 kb).

彩新网_[官网首页]Lucigen独特的克隆试剂盒带有经过预处理的载体和高效能的细胞,可避免数小时的繁琐准备工作并实现高效克隆。结扎仅需30分钟即可完成,无需清理;转变前只需要短暂的热变性即可��zui小的载体大小(1.7-2.0 kb)有助于下游操作(例如诱变,随后的插入片段添加)。

Stabilized Inserts and Gap-Free Cloning

All cloning kits include the transcription- and translation-free pSMART® vectors (Figure 1), which eliminate many of the problems associated with cloning recalcitrant DNA in conventional plasmid vectors. Conventional cloning vectors contain promoters (e.g., lacZ promoter) that constitutively transcribe the insert sequence. This transcription and coupled translation can destabilize inserts that contain coding regions, strong promoters, short repeats, or incompatible secondary structures. The cloning site of pSMART vectors does not include a lacz sequence. In addition, strong transcription terminators flank the cloning site to block spurious transcription from the vector and insert-driven transcription into the vector. Low copy number versions of pSMART further stabilize sequences that are difficult to maintain in typical vectors.

所有克隆试剂盒均包含无转录和翻译的pSMART®载体(图1),从而消除了与克隆常规质粒载体中的顽固性DNA有关的许多问题。彩新网_[官网首页]常规的克隆载体包含组成性转录插入序列的启动子(例如lacZ启动子)。这种转录和偶联翻译可破坏包含编码区,强启动子,短重复序列或不相容二级结构的插入片段的稳定性。pSMART载体的克隆位点不包含  lacz序列。另外,强转录终止子位于克隆位点的侧面,以阻止来自载体的假转录和插入驱动的转录到载体中。pSMART的低拷贝数版本进一步稳定了难以在典型载体中维持的序列。

No Background Problems

The pSMART vectors are supplied pre-digested, with blunt, dephosphorylated ends, and are qualified to produce 99.5% recombinant clones in typical experiments. The ultra-low background of empty vector (less than 0.5%) eliminates the need to screen for recombinants, removes the uncertainty of false negatives (light blue pUC colonies) and false positives (white colonies that lack inserts), and enables library construction from nanogram amounts of DNA.

彩新网_[官网首页]pSMART载体预先消化,末端钝化,去磷酸化,在典型实验中有资格生产99.5%的重组克隆。空载体的超低背景(小于0.5%)消除了筛选重组子的需要,消除了假阴性(浅蓝色pUC菌落)和假阳性(缺少插入物的白色菌落)的不确定性,并能够从纳克量的DNA。

Figure 1. High copy (HC) and low copy (LC) versions of the pSMART transcription-free cloning vectors.

图1.  pSMART无转录克隆载体的高拷贝(HC)和低拷贝(LC)版本。

Convenient Success

Efficiently obtain your clone or create your genomic or cDNA library—the CloneSmart Cloning Kits eliminate tedious preparation of vectors and competent cells, as well as time-consuming QC testing experiments. Kits include optimized reagents, ligation-ready vector (no post-ligation cleanup step required), detailed instructions, and trouble-shooting guides to simplify cloning and sequencing. Highly efficient E. cloni® 10G electrocompetent cells (up to >4 × 1010 cfu/µg) or chemically competent cells ( >1 × 109 cfu/µg) are available for sale separately.

有效地获得您的克隆或创建您的基因组或cDNA文库-CloneSmart Cloning Kits消除了繁琐的载体和感受态细胞制备,以及费时的QC测试实验。试剂盒包括优化的试剂,可用于连接的载体(无需连接后清chu步骤),详细说明以及用于简化克隆和测序的故障排除指南。单独提供高效的  大肠杆菌克隆 10G电感受态细胞(zui高> 4×1010 cfu / µg)或化学感受态细胞(> 1×109 cfu / µg)。

 
lucigen 40300-4 40 rxns CloneSmart® LCAmp Blunt Cloning Kit 列表数据
Contact your local distributor for pricing
Product Description Size Cat. No.
CloneSmart HCAmp Blunt Cloning Kit   20 rxns 40041-2
    40 rxns 40041-4
CloneSmart HCKan Blunt Cloning Kit   20 rxns 40704-2
    40 rxns 40704-4
CloneSmart LCAmp Blunt Cloning Kit   20 rxns 40300-2
    40 rxns 40300-4
CloneSmart LCKan Blunt Cloning Kit   20 rxns 40821-2
    40 rxns 40821-4
产品描述 尺寸 彩新网_[官网首页]猫。没有。
CloneSmart HCAmp Blunt克隆试剂盒   20个 40041-2
    40 rxns 40041-4
CloneSmart HCKan Blunt克隆试剂盒   20个 40704-2
    40 rxns 40704-4
CloneSmart LCAmp Blunt克隆试剂盒   20个 40300-2
    40 rxns 40300-4
CloneSmart LCKan Blunt克隆试剂盒   20个 40821-2
    40 rxns 40821-4

ORDER INFORMATION
订购信息
彩新网_[官网首页]CloneSmart Blunt克隆试剂盒包含:载体预混液,CloneSmart DNA连接酶,阳性对照插入DNA,测序引物和完整的实验方案。 试剂盒可用于任何具有  化学感受态或电感受态能力的大肠杆菌,但已针对与E.cloni®  感受态细胞和化学感受态细胞一起使用进行了优化  
CloneSmart Blunt Cloning Kits contain: Vector Premix, CloneSmart DNA Ligase, Positive Control Insert DNA, Sequencing Primers, and a complete protocol. Kits can be used with any 
E. coli chemically competent or electrocompetent cells but have been optimized for use with of E. cloni® Electrocompetent Cells and Chemically Competent Cells.

对此产品有兴趣,可联系中北林格获取更多详细信息。 

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